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Product Info Summary
| SKU: | M00227-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Equine |
| Host: | Mouse |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody
SKU/Catalog Number
M00227-3
Size
100ul
Description
Boster Bio Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody catalog # M00227-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Equine.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-3)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Full length protein purified from pig blood cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00227-3 is reactive to GAPDH in Equine
Applications
M00227-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
36.053kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000 IF/IHC: 1:100.
Validation Images & Assay Conditions
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Western blot analysis of cell line lysates probed with mouse mAb to GAPDH, M00227-3, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a “house keeping” protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
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Immunofluorescent analysis of of HeLa cells stained with mouse mAb to GAPDH, M00227-3, dilution 1:100 in green. Blue is DAPI staining of nuclear DNA. The M00227-3 antibody produces strong cytoplasmic staining of healthy cells.
Protein Target Info & Infographic
Gene/Protein Information For GAPDH (Source: Uniprot.org, NCBI)
Gene Name
GAPDH
Full Name
Glyceraldehyde-3-phosphate dehydrogenase
Weight
36.053kDa
Superfamily
glyceraldehyde-3-phosphate dehydrogenase family
Alternative Names
1D4 GAPDH; aging-associated gene 9 protein; Cytoplasm Marker; EC 1.2.1; EC 1.2.1.12; EC 2.6.99.-; G3PD; G3PD; G3PDH; GAPD; GAPDH; GAPDPeptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38; OCT1 coactivator in S phase; Peptidyl-cysteine S-nitrosylase GAPDH GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GAPDH, check out the GAPDH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAPDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody (M00227-3)
Hello CJ!
M00227-3 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
H2S attenuates the myocardial fibrosis in diabetic rats through modulating PKC-ERK1/2MAPK signaling pathway Junrong Long 1 1, Maojun Liu 1, Shengquan Liu 1, Feng Tang 1, Wenting Tan 1, Ting Xiao 1, Chun Chu 2, Jun Yang 1 Technol Health Care. 2019;27(S
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